Facsdiva manual

Manual facsdiva

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Only PerCP channel should display a positive peak in the PerCP single-stained tube. This manual contains reference information about the BD FACSCanto™ II flow cytometer and the BD FACS™ Loader. For In Vitro Diagnostic Use bdbiosciences. For more information, see Working With Snap-To Gates in. 0 Reference Manual Stanford.

Page 136: Setting Up The Workspace. The BD FACSDiva Software Reference Manual assumes you have a working knowledge of basic Microsoft® Windows® operation. BD FACSDiva software provides new features to help users integrate flow systems into new application areas, including index sorting for stem cell and single-cell applications, as well as automation protocols for high-throughput and robotic laboratories. BD FACSDiva Operation Manual – Spectral Overlap & Compensation - MANUAL 4 i.

For more detailed instructions on how to use the software, refer to the BD FACSDiVa Software User’s Guide. Refer to the BD FACSDiva Software Reference Manual for details. In addition, the Auto-Size and Auto-Movement featur es can now be used with Snap-To Interval gates.

See Dragging Gates into the Population Hierarchy on page 232 in the BD FACSDiva Software Reference Manual. BD FACSDiva Software Quick Reference Guide for Administrators (Manual) This guide contains instructions for using BD FACSDiva™ software version8. Not for use in diagnostic or therapeutic procedures. 0 data: 18 bit resolution (262,144 channels). 0 not FCS2 (important, since it preserves below-zero values).

Wait 10 seconds, then Open the BD FACSDiva software and log on. Most instrument functions are controlled by BD FACSCanto™ clinical software and BD FACSDiva™ software. If machine is on, depressurize. ) cb336 at leicester. Note that plots with a colored background are now printed in the specified color. Previous message: Cytometry AriaII with 130 um nozzle Next message: Cytometry stem cells FC block Messages sorted by:.

Older BD FACSDiva versions – you may sometimes wish to export as Experiment. • Turn off the cytometer main power. In addition, a printed copy can be requested from BD Biosciences.

LSR II Quick Reference & Troubleshooting Guide Startup Ensure that you signed up for instrument use using the on-line calendar (even if it is night or weekend). If you are the first user of the day: Boot up the computer and log on to Windows. controlled by BD FACSDiVa™ software, this guide also contains basic software information needed for instrument setup. Join our mailing list for the latest news, updates and more. For instructions on running a complete fluidics shutdown, refer to the Fluidics Shutdown section in the BD FACSAria II User’s Guide. † New feature to apply gate coordinates (such as shape and size) between worksheets to other tubes in an open experiment.

• Snap-To Interval gates can now be tethered to manual gates. FACSDiVa (BD Biosciences). Refer to the BD FACSDiva facsdiva manual Software Reference Manual for ideas on how to organize experiments. BD FACSDiva Operation Manual – Spectral Overlap & Compensation -AUTO 1 BD FACSDiva: Standard Operation Protocol Spectral Overlap & Compensation – PROGRAMME-AIDED User should understand the Basic Operation before proceeding to the following steps. For Research Use Only.

BD FACSDiva Software Basic Experiment Guide Please note, this guide is not intended to take the place of in-person training but is to be used as a reference as needed after completing training with Unified Flow Core staff. A May BD Biosciences 2350 Qume Drive San Jose, CAUSA TelFaxBD FACSDiva Software. Equipment Documentation Applied Precision DeltaVision Core DeltaVision Core Specs Olympus IX71 Microscope Stand User’s Manual Photometrics CoolSNAP HQ2 WeatherStation Environmental Chamber Manual BD FACSAria II Flow Cytometer BD FACSAria II Website FACSAria II Tech Specs BD/Cytopeia Influx Flow Cytometer Influx User Guide (ISB) Influx Quick-start Operation (ISB) Influx Operator Manual BD. For the demonstration, we will assume this is a 4-color flow cytometric analysis. in the BD FACSDiva Software Reference Manual. Upon startup, the software will connect to the cytometer. The BD FACSCanto Flow Cytometer Reference Manual assumes you have a.

See the online manual and the basic and advanced training video recorded on June 15th and 16th for CCHMC on FlowJo v10. See more results. to the FACSDiVa software manual and may The last step is to load the 96 FCS files into FlowJo FlowJo Compensation BD FACSDivaв„ў Software Reference Manual, Bd Facsdiva Software 7. It can be accessed from the BD FACSDiva Software Help menu (Help > Literature > Reference Manual), or by double-clicking the shortcut on the desktop. It is extremely important to read and understand. The facsdiva format of the user name for FACSDiva software is the first letter of your first name followed by your full last name. Compensation Controls in the BD FACSDiva Software Reference Manual.

Access this manual from the BD FACSDiva Software Help menu (Help > Literature > Reference Manual), or by double-clicking the shortcut on the desktop. x can export data two ways; “Export Experiment” or “Export FCS files”. Refill as necessary from large plastic canister containing 1x sheath solution.

A full tank provides about 3 hours’ run time. • Biexponential display is enabled by default, but can be disabled in user preferences, allowing more events to be recorded per experiment. % value under Spectral Overlap column should be increased until those ‘false positive’ peaks in all other channels shift to their pre-defined negative region. Watch a video overview of FACSDiVa; FCSExpress (DeNovo Software). Biexponential Display The biexponential display feature has been enhanced as follows.

We highly recommend exporting as digital FCS3. Operating instructions are contained in the BD FACSCanto II Instructions for Use. z BD FACSDiva Software Reference Manual: Includes instructions or descriptions for installation and setup, workspace components, acquisition controls, analysis tools, and data management. Some of the older versions transposed facsdiva manual the compensation matrix. The BD FACSAria User’s Guide assumes you have a working knowledge of basic Microsoft® Windows® operation.

Cytometry How to recover deleted FACSDiva files Boes, Christoph (Dr. Diagrams and drawings will be included to assist in the description of components and layout of the ProWriter TM F 0. 8 Check connectivity status at the bottom of the Instrument window to verify that the software connects to the cytometer. BD Facsdiva Software 6. Acquire your CBA on FACSDiva as per manufacturer instructions (ie like a normal FACS expt).

† BD FACSDiva Software Reference Manual: Includes instructions or descriptions for installation and setup, workspace components, acquisition controls, analysis tools, and data management. GETTING STARTED 1. If this is part of a daily shutdown, perform these steps. † Drag and drop gates in the population hierarchy. BD FACSDiva Software Quick Reference Guide for the BD LSR II Subject: This guide contains instructions for using BD FACSDiva software&92;r version 6. Serial Number: VSoftware: BD FACSDiva 6.

For instructions on creating a user name and password, refer to the BD FACSDiva Software Reference Manual. 0 and later with BD LSR II flow cytometers. 3 Input Device: Manual Date: 01:39 PM Cytometer Configuration: Pharmingen 3-laser, 8-colorSetup Beads Bead Product: CST Setup Beads, Part : 910723 Lot ID: 54102, Expiration Date: Bead Lot Information: Available Detector Settings Laser Detector Parameter PMTV New. ModFit LT (Verity Software House). Current BD FACSDiva version 6. BD Biosciences recommends that first-time user’s of this instrument take advantage of operator training offered with the sale of every new instrument. The BD FACSDiva Software Reference Manual assumes that you have a working knowledge of basic Microsoft® Windows® operation. In the login window, scroll down through the list of user names to locate yours.

Wait 10 seconds, facsdiva manual then. · The first, oddly enough, is BD&39;s very own Index Sorting Manual ( Documentation > Reference Manual), or by double-clicking the shortcut on the desktop.

Facsdiva manual

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